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chromatographic analysis

(krō-măt″ă-grăf′ĭk ă-năl′ĭ-sĭs) Analysis of substances on the basis of the reaction of the constituents as they are differentially absorbed on one of a variety of materials such as filter paper.


(krō″mă-tŏg′ră-fē) [chrom- + -graphy] The separation of two or more chemical compounds in a liquid or gaseous mixture by their removal at different rates based on differential solubility and adsorption. This separation is often accomplished by letting the chemicals percolate through a column of a powdered adsorbent or by passing them across the surface of an adsorbent paper, among other techniques. chromatographic (mat″ŏ-graf′ik), adj.

adsorption c. Chromatography accomplished by applying the test material to one end of a sheet or column containing a solid. As the material moves, the various constituents adhere to the surface of the particles of the solid at different distances from the starting point according to their chemical characteristics.

column c. A form of adsorption chromatography in which the adsorptive material is packed into a column.

gas c. An analytical technique in which a sample is separated into its component parts between a gaseous mobile phase and a chemically active stationary phase.

gas-liquid c. ABBR: GLC. Chromatography in which a gas moves over a liquid, and chemical substances are separated on the liquid by their different adsorption rates.

gel filtration c. A type of column chromatography in which chemicals are separated via pores according to their molecular size.

high-performance liquid c. ABBR: HPLC. Application of high pressure to liquid chromatography technique to increase separation speed and enhance resolution. SYN: high pressure liquid c.

high pressure liquid c. High-performance liquid chromatography.

paper c. Chromatography in which paper strips are used as the porous solid medium.

partition c. Chromatography in which substances in solution are separated by being exposed to two immiscible solvents. The immobile solvent is located between the spaces of an inert material such as starch, cellulose, or silica. The substances move with the mobile solvent as it passes down the column at a rate governed by their partition coefficient.

thin-layer c. ABBR: TLC. Chromatography involving the differential adsorption of substances as they pass through a thin layer or sheet of cellulose or some other inert compound.


(krō′mă-toyd) [Gr. chroma, color, + eidos, form, shape] Staining in the same manner as chromatin.


(krō″mă-tō-kī-nē′sĭs) [″ + kinesis, movement] The movement of chromatin during the division of a cell.


(krō″mă-tŏl′ĭ-sĭs) [″ + lysis, dissolution] The dissolution of chromophil substance (Nissl bodies) in neurons in certain pathological conditions, or following injury ...

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